Determination of Selenium in Biological Samples by Hydride Generation- Atomic Fluorescence Spectrometry

A method for determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry was developed. The samples were decomposed with HNO₃-HClO₄ and Fe³⁺ was used as relieving reagent in 4 mol/L HCl medium. The detection limit of the method was 0.02 μg/L. The method has been applied to the determination of selenium in biological national standard reference materials and the results are in agreement with certified values with precision of 1.51% RSD(n=11).