高效液相色谱中有效理论塔板数的变量因素和柱效率的研究

A Study on Variable Factors of Effective Theoretical Plate Number and Column Efficiency in High Performance Liquid Chromatography

摘要: 研究了高效液相色谱（ＨＰＬＣ）、高效离子色谱（ＨＰＩＣ）、高效离子排斥色谱（ＨＰＥＩＣ）和低压离子色谱（ＬＰＩＣ）中有效理论塔板数的变量因素，提出了能定量表述柱分离效率的参数──柱效率Ｓ及其计算方法。给出了其它色谱参数（分离度Ｒｓ，分离系数α和容量因子Ｋ＇）与有效理论塔板数及柱效率相关的实验结果。

Abstract: The variable factors which influence the effective theoretical plate number in HPLC, HPIC,HPEIC and LPIC are discussed in this paper."S"(Column efficiency),a new parameter which can express the column separation efficiency quantitatively and its calculati