【引用本文】 程雅楠, 关翔宇, 曲文龙, 等. 氨氮污染源区包气带剖面微生物生态分布特征的研究[J]. 岩矿测试, 2013, 32(2): 290-299.
CHENG Ya-nan, GUAN Xiang-yu, QU Wen-long, et al. Microbial Ecological Diversity Characteristics of the Soil Profile in the Vadose Zone Polluted by Ammonia Nitrogen[J]. Rock and Mineral Analysis, 2013, 32(2): 290-299.

氨氮污染源区包气带剖面微生物生态分布特征的研究

1. 

水资源与环境工程北京市重点实验室,中国地质大学(北京),北京 100083

2. 

中交铁道勘察设计院有限公司,北京 100088

3. 

沈阳水务集团,辽宁 沈阳 110003

收稿日期: 2012-03-07  接受日期: 2012-07-24

基金项目: 国家自然科学基金项目面上基金(51078338);中央高校基本科研业务费自由探索项目(2010ZY05)

作者简介: 程雅楠,硕士研究生,从事地下水及土壤污染微生物修复技术研究。E-mail:ptinny_5696@163.com。

通信作者: 陈鸿汉,教授,主要从事地下水科学与环境工程教学与科研工作。E-mail:chenhonghan@cugb.edu.cn

Microbial Ecological Diversity Characteristics of the Soil Profile in the Vadose Zone Polluted by Ammonia Nitrogen

1. 

Beijing Key Laboratory of Water Resources and Environmental Engineering, China University of Geosciences (Beijing), Beijing 100083, China

2. 

Railway Survey and Design Institute of China Communications Co. LTD, Beijing 100088, China

3. 

Shenyang Water Group Co. LTD, Shenyang 110003, China

Corresponding author: CHEN Hong-han, chenhonghan@cugb.edu.cn

Received Date: 2012-03-07
Accepted Date: 2012-07-24

摘要:包气带土壤中氨氮污染迁移规律已成为近年来国内外学者研究的重点,目前在氨氮污染迁移规律研究中采用柱实验模拟以及软件模拟的方法较多,土壤生物技术则广泛应用于污染物降解领域,而在污染物迁移规律研究中应用较少。本文采用变性梯度凝胶电泳(DGGE)技术、16S rRNA序列分析技术以及典范对应分析相结合,对华北平原3个典型氨氮污染区土壤表层至包气带剖面微环境的细菌垂直分布特征及群落结构进行研究。结合污染区土壤理化性质分析,认为包气带土壤剖面的细菌群落中存在着与氮循环、硫酸盐代谢等过程偶联的优势细菌类群,说明土壤微环境中细菌群落分布明显受氨态氮、硝态氮、亚硝态氮和硫酸盐的分布影响,进一步表明污染土壤优势菌群的群落结构信息是描述包气带土壤环境氨氮污染物迁移规律的重要参数。

关键词: 包气带, 氨氮污染, 微生物群落, 变性梯度凝胶电泳, 16S rRNA

Microbial Ecological Diversity Characteristics of the Soil Profile in the Vadose Zone Polluted by Ammonia Nitrogen

KEY WORDS: vadose zone, ammonia pollution, microbial communities, Denaturing Gradient Gel Electrophoresis (DGGE), 16S rRNA

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氨氮污染源区包气带剖面微生物生态分布特征的研究

程雅楠, 关翔宇, 曲文龙, 陈鸿汉, 刘菲, 谢宇轩, 朱玲玲