【引用本文】 徐红纳, 靳立国, 由丽梅, 等. 一阶导数分光光度法同时测定水样中Cr(Ⅲ)和Cr(Ⅵ)[J]. 岩矿测试, 2020, 39(5): 790-797. doi: 10.15898/j.cnki.11-2131/td.201908090118
XU Hong-na , JIN Li-guo , YOU Li-mei , et al. Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method[J]. Rock and Mineral Analysis, 2020, 39(5): 790-797. doi: 10.15898/j.cnki.11-2131/td.201908090118

一阶导数分光光度法同时测定水样中Cr(Ⅲ)和Cr(Ⅵ)

1. 牡丹江医学院, 黑龙江 牡丹江 157011;

2. 黑龙江省第一地质勘查院, 黑龙江 牡丹江 157011

收稿日期: 2019-08-09  修回日期: 2019-12-11 

基金项目: 2019年度黑龙江省省属高等学校基本科研业务费科研项目(2019-KYYWFMY-0055)

作者简介: 徐红纳,硕士,讲师,主要从事分析化学研究。E-mail:13838782@163.com。。

Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

1. Mudanjiang Medical University, Mudanjiang 157011, China;

2. The First Geological Prospecting Institute of Heilongjiang Province, Mudanjiang 157011, China

Received Date: 2019-08-09
Revised Date: 2019-12-11

摘要:环境水样中的铬通常以Cr(Ⅲ)和Cr(Ⅵ)的形态存在,不同价态的铬产生不同的生理作用,对不同价态铬进行准确分析是必要的。目前Cr(Ⅲ)和Cr(Ⅵ)的测定方法大多数是先分离后再测定,或先测定出Cr(Ⅲ)或Cr(Ⅵ),然后通过氧化或还原测定铬的总量,再差减法计算出另一价态铬的含量,此操作复杂,并且在处理过程中易导致价态的改变,误差较大,精确度难以保证。本文用一阶导数分光光度法消除了Cr(Ⅲ)对Cr(Ⅵ)干扰,能同时测定Cr(Ⅲ)或Cr(Ⅵ)的含量。混合水样中加入显色剂EDTA-2Na,调节溶液pH在3~3.5,恒温水浴70℃条件保持加热15min,测定吸光度,导数间隔因子为10nm求一阶导数,在波长330nm处Cr(Ⅵ)吸光度一阶导数有最大值,而在此波长处Cr(Ⅲ)-EDTA络合物吸光度一阶导数值为0,可用一阶导数分光光度法测定Cr(Ⅵ)的含量,在Cr(Ⅲ)-EDTA的最大吸收波长543nm处测溶液吸光度,直接测定Cr(Ⅲ)的含量。在优化实验条件下,Cr(Ⅲ)线性回归方程为A=0.0036ρ-0.0002(r2=0.9999),线性范围为0~120mg/L,检出限为0.006mg/L;Cr(Ⅵ)线性回归方程为D=0.00072ρ-0.00013(D为一阶导数值,r2=0.9996),线性范围为0~100mg/L,检出限为0.005mg/L。Cr(Ⅲ)和Cr(Ⅵ)加标回收率为97.8%~102.6%。该方法能够满足废水中Cr(Ⅲ)和Cr(Ⅵ)分析测试要求。

关键词: 水样, Cr(Ⅲ), Cr(Ⅵ), 同时测定, 导数间隔因子, 一阶导数分光光度法

Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

KEY WORDS: water sample, Cr (Ⅲ), Cr (Ⅵ), simultaneous determination, derivative interval factor, first derivative spectrophotometry

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一阶导数分光光度法同时测定水样中Cr(Ⅲ)和Cr(Ⅵ)

徐红纳, 靳立国, 由丽梅, 程艳