【引用本文】 陈金保, 柳英霞, 李绪涛, 等. 高效液相色谱-串联质谱法同时测定食用植物油中多种有机锡化合物[J]. 岩矿测试, 2017, 36(2): 180-186. doi: 10.15898/j.cnki.11-2131/td.2017.02.012
CHEN Jin-bao, LIU Ying-xia, LI Xu-tao, et al. Determination of a Variety of Organotin Compounds in Edible Vegetable Oil by Liquid Chromatography-Tandem Mass Spectrometry[J]. Rock and Mineral Analysis, 2017, 36(2): 180-186. doi: 10.15898/j.cnki.11-2131/td.2017.02.012

高效液相色谱-串联质谱法同时测定食用植物油中多种有机锡化合物

1. 

江西省地质调查研究院, 江西 南昌 330030

2. 

南昌大学分析测试中心, 江西 南昌 330047

收稿日期: 2016-02-29  修回日期: 2017-12-27  接受日期: 2017-01-10

基金项目: 江西省重点研发计划项目(20161BBG70063)

作者简介: 陈金保, 教授级高级工程师, 主要从事地质实验测试。E-mail: dkchenjb@163.com。

通讯作者: 柳英霞, 博士, 实验师, 研究方向为食品质量与安全。E-mail: liuyingxia@ncu.edu.cn

Determination of a Variety of Organotin Compounds in Edible Vegetable Oil by Liquid Chromatography-Tandem Mass Spectrometry

1. 

Jiangxi Institute of Geological Survey, Nanchang 330030, China

2. 

Center of Analysis and Testing, Nanchang University, Nanchang 330047, China

Corresponding author: LIU Ying-xia, liuyingxia@ncu.edu.cn

Received Date: 2016-02-29
Revised Date: 2017-12-27
Accepted Date: 2017-01-10

摘要:有机锡化合物已广泛应用于食品包装材料及其生产过程中,对人体健康存在着潜在的危害。特别是食用植物油因甘油三酯(脂肪)含量较高,约占植物油的95%以上,给分析检测带来较大的基体干扰。本文采用低温冷冻提取结合固相萃取技术,建立了高效液相色谱-串联质谱法同时测定食用植物油样品中二丁基二氯化锡、二苯基二氯化锡、三丁基氯化锡、三苯基氯化锡同时分析的方法。实验对比了甲醇、乙腈、乙醇三种有机萃取溶剂分别在低温冷冻提取和常规液液振荡提取条件下的提取效率,并考察了PSA、中性氧化铝、弗罗里硅土及活性炭四种净化材料对食用油提取液的净化效果。确定了食用油中有机锡化合物的最佳提取净化方案为:以10 mL甲醇为提取溶剂在低温冷冻条件下充分提取两次,并通过50 mg活性炭净化提取液。为评价该方法的准确性,对食用油样品进行三个不同水平的添加回收实验,各有机锡化合物的平均回收率在74.2%~99.5%之间,接近或优于现行国标及相关研究(70%~120%),可应用于菜籽油、花生油、芝麻油、大豆油、玉米油及调和油等不同基质的食用植物油中有机锡化合物的同时测定。

关键词: 有机锡化合物, 高效液相色谱-串联质谱法, 食用植物油, 低温冷冻提取, 分散固相萃取

Determination of a Variety of Organotin Compounds in Edible Vegetable Oil by Liquid Chromatography-Tandem Mass Spectrometry

KEY WORDS: organotins compounds, Liquid Chromatography-Tandem Mass Spectrometry, edible vegetable oils, dispersive-solid phase extraction, low-temperature precipitation extraction

Highlights

· The organotins in the edible vegetable oil were extracted successfully by the low-temperature precipitation process, and a large amount of lipid was removed effectively.

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高效液相色谱-串联质谱法同时测定食用植物油中多种有机锡化合物

陈金保, 柳英霞, 李绪涛, 郭岚