Abstract: The paper presents the simulation experiment using semi-permeable sampling device(SPMD) with triolein in order to develop a method to monitor groundwater by simulating bio-accumulation process. The uptake rate and equilibrium time of organochlorine pesticides were obtained during the laboratory experiment. All the equilibrium time are 594 hours except that δ-BHC′s is 234 hours and both p,p′-DE and o,p-DT′s are 70 hours. Uptake rates of α-HC, hexachlorobenzene, β-HC, γ-HC, δ-HC, heptachlor, aldrin, heptachlor epoxide, p,p′-DDE, dieldrin, endrin, p,p′-DD, o,p-DT and p,p′-DT are 0.061, 0.071, 0.098, 0.066, 0.033, 0.014, 0.007, 0.062, 0.108, 0.052, 0.029, 0.032, 0.061 mL/h, respectively. Because the concentrations of heptachlor, aldrin, o,p-DDT, p,p′-DDE and p,p′-DDT in SPMD are lower than those in water, these contaminants are not suitable to be sampled by this SPMD. The results of field experiment done at the Liangshui river prove the feasibility of SPMD used in groundwater monitoring. But detailed work is needed to be done in order to explain some unusual field experiment results.