Abstract: The functions, characteristics and applications of laboratory information management system (LIMS)—Geo-LIMS 2.0 developed by Wuhan Institute of Geology and Minerals Resources were introduced and discussed in detailed in this paper. From the actual work of mineral laboratory and combining the information technology with existing quality assurance system of laboratory, the automatic processing of computer information has achieved in the whole process of sample login, task assignment, job generation, data recording and computing, data collection, quality control and report generation in Geo-LIMS 2.0. The process covers the main aspects of laboratory management work. The operational streamline and working procedure are simplified and optimized. It makes benefits for managers and analysts to more focus on development of core analytical technology, greatly improving the efficiency and quality of work. Geo-LIMS 2.0 has the advantage of small data entry, simple operation, powerful function of data query and statistics, perfect rights management, simple and rapid system maintenance to meet the requirements of geological and mineral laboratory management.