【引用本文】 董会军, 董建芳, 王昕洲, 等. pH值对HPLC-ICP-MS测定水体中不同形态砷化合物的影响[J]. 岩矿测试, 2019, 38(5): 511-518. doi: 10.15898/j.cnki.11-2131/td.201808230096
DONG Hui-jun , DONG Jian-fang , WANG Xin-zhou , et al. Effect of pH on Determination of Various Arsenic Species in Water by HPLC-ICP-MS[J]. Rock and Mineral Analysis, 2019, 38(5): 511-518. doi: 10.15898/j.cnki.11-2131/td.201808230096

pH值对HPLC-ICP-MS测定水体中不同形态砷化合物的影响

河北省地质环境监测院, 河北省地质资源环境监测与保护重点实验室, 河北 石家庄 050021

收稿日期: 2018-08-23  修回日期: 2019-03-09 

基金项目: 河北省地矿局地质科技发展项目(201610);河北省“三三三人才工程”(A2017002031)

作者简介: 董会军,工程师,主要从事地下水检测工作。E-mail:donghuijun@hegeoenv.com。。

通信作者:

Effect of pH on Determination of Various Arsenic Species in Water by HPLC-ICP-MS

Hebei Geological Environment Monitoring Institute, Hebei Key Laboratory of Geological Resources and Environment Monitoring and Protection, Shijiazhuang 050021, China

Corresponding author: WANG Xin-zhou , 主要从事地下水监测、地质灾害预警等工作。jcywxz@hegeoenv.com。

Received Date: 2018-08-23
Revised Date: 2019-03-09

摘要:在高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)测定不同形态砷化合物的检测技术中,流动相的pH值是一个关键影响因素。以往较多的研究仅关注了pH值对分离度的影响,通过比较分离度的优劣选择一个适用的pH值。本文采用HPLC-ICP-MS技术测定不同形态的砷化合物,以30mmol/L碳酸氢铵溶液作为流动相,研究了当流动相pH值在6.0~9.7范围内变化时,对砷形态化合物保留时间和峰强度产生的影响,从砷化合物的分离度、灵敏度和分析成本三个方面确定合理的分析方案。结果表明:pH值在6.0~7.5和9.5~9.7范围内,As(Ⅲ)、As(Ⅴ)、一甲基砷(MMA)和二甲基砷(DMA)四种形态的砷化合物的色谱峰分离效果较好;pH值在8.0~9.0范围时,随着pH值的增加,As(Ⅲ)和DMA出峰顺序发生了交换;pH为弱酸性时,砷化合物的灵敏度均较高;pH为偏碱性时,分析周期变短,适用于快速分析。碱性条件下,部分砷化合物与色谱柱结合能力更强,被保留在色谱柱内,使得色谱柱的寿命可能缩短。研究认为:偏酸性的流动相条件,适用于超低浓度(如≤ 10μg/L)的样品分析;偏碱性的流动相条件,具有明显的时间成本和运行成本优势,但要对色谱柱定期维护。

关键词: 水体, 砷形态, pH值, 高效液相色谱-质谱法, 分离度, 灵敏度, 分析成本

Effect of pH on Determination of Various Arsenic Species in Water by HPLC-ICP-MS

KEY WORDS: water, arsenic species, pH, high-performance liquid chromatography-inductively coupled plasma-mass spectrometry, separation, sensitivity, analysis cost

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pH值对HPLC-ICP-MS测定水体中不同形态砷化合物的影响

董会军, 董建芳, 王昕洲, 李义, 赵峰